phase-contrast microscope nikon eclipse te-200-u  (Nikon)

Journal: Oncotarget

Article Title: MicroRNA 603 acts as a tumor suppressor and inhibits triple-negative breast cancer tumorigenesis by targeting elongation factor 2 kinase

doi: 10.18632/oncotarget.14264

Figure Lengend Snippet: A . The silencing of eEF2K by siRNA (50 nM) significantly reduced the number of colonies formed by MDA-MB-231 and MDA-MB-436 cells (*** p < 0.001). Cells were transfected every 4 days with the control or eEF2K siRNA. B . MDA-MB-231 and MDA-MB-436 cell invasion was assessed by using a matrigel transwell assay. Cells were transfected with eEF2K siRNA or control siRNA or not transfected (NT)); after 72-h transfection, the cells were transferred to transwell and incubated for 24 h and cells invading matrigel and passing through the membrane we counted by light microscope. C . Western blot analysis of p-EF2 Thr56 , p-Src Tyr416 , total Src, p-Fak Tyr397 , total Fak, p-Akt Ser473 , total Akt and cyclin D1 in the indicated cells. β-Actin was used as a loading control.

Article Snippet: After the treatments, the cells were photographed using a phase contrast microscope (Nikon Eclipse TE-200-U) to determine the wound width at 0 h. The cultures were continued, and images were captured at 0 and 36h instead of 12, 24, and 48h.

Techniques: Transfection, Control, Transwell Assay, Incubation, Membrane, Light Microscopy, Western Blot